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SDS-PAGE - University of California Davis

SDS-PAGE - University of California Davis

Aug 18, 2003 2O 1.8 ml 3.6 ml 1.6 ml 3.2 ml 1.5 M Tris-HCl, pH 8.8 (RG Bfr.) 1.3 ml 2.6 ml 1.3 ml 2.6 ml 40% Acrylamide Stock 800 l 1.6 ml 1.0 ml 2.0 ml 20 % SDS 100 l 200 l 100 l 200 l 30% Ammonium Persulfate 10 l 20 l 10 l 20 l TEMED 4 l 8 l 4 l 8 l 12 % 15% 1 gel 2 gels 1 gel 2 gels DDI H

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SARS CoV 2 Spike Antibody IgG - University of Washington

SARS CoV 2 Spike Antibody IgG - University of Washington

Centrifuge GOLD SST tube and route to Eastlake Virology (EVIR rack 81). Centrifuge RED TOP or EDTA tube and aliquot serum/plasma into plastic aliquot tube. Route to Eastlake Virology (EVIR rack 81). Stability: Sample stable off the clot, red blood cells, or …

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COVID-19 Specimen Submission Guidelines coronavirus

COVID-19 Specimen Submission Guidelines coronavirus

Collect at least 5 mL of blood in a plastic serum separator tube (SST). Gold top tubes with gel serum separator, preferred. Centrifuge the SST according to blood collection manufacturer's instructions for separation of serum from whole blood. Please refrigerate SST at 2-8ᵒC after centrifuging the specimen.

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DMSO Hybri-Max sterile-filtered CAS No. 67-68-5 Sigma-Aldrich

DMSO Hybri-Max sterile-filtered CAS No. 67-68-5 Sigma-Aldrich

Dimethyl sulfoxide (DMSO); Hybri-Max, sterile-filtered, BioReagent, hybridoma, = 99.7%; Suitable for hybridoma cell culture; DMSO is a polar aprotic solvent used in chemical reactions, in polymerase chain reactions (PCR) and as a cryoprotectant vitrification agent for …

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Disc Stack Centrifuge - an overview ScienceDirect Topics

Disc Stack Centrifuge - an overview ScienceDirect Topics

Example 11.2. Cell Recovery in a Disc Stack Centrifuge. A continuous disc stack centrifuge is operated at 5000 rpm for separation of bakers’ yeast. At a feed rate of 60 l min −1, 50% of the cells are recovered. For operation at constant centrifuge speed, solids recovery is inversely proportional to the flow rate. (a)

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Tubes - Sarstedt

Tubes - Sarstedt

How exact is the scale on the 15 ml and 50 ml tubes? The scale on the 15 ml tubes has a volume tolerance of approx. +/- 100 l, while the tolerance on the 50 ml tubes is +/- 250 l. What are the maximum speeds at which the reagent and centrifuge tubes can be centrifuged?

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Laboratory Centrifuges Biocompare

Laboratory Centrifuges Biocompare

Microcentrifuges are staple instruments in many research laboratories that generally accommodate small tube volumes such as 2 mL, 1.5 mL, 0.5 mL and PCR tubes. Microcentrifuges for routine laboratory procedures typically spin at speeds up to 16,000 x g, while more specialized instruments can reach speeds up to 30,000 x g.

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How to Use a Centrifuge: 14 Steps (with Pictures) - wikiHow

How to Use a Centrifuge: 14 Steps (with Pictures) - wikiHow

Nov 03, 2020 A centrifuge is a type of research equipment that spins a liquid suspension at high rotation rates to separate it into distinct layers based on density. Because of these high rotation rates, centrifuges are delicate, can break easily, and...

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Mens Fleece Jackets -

Mens Fleece Jackets -

Shop the best selection of men's fleece jackets at Backcountry.com, where you'll find premium outdoor gear and clothing and experts to guide you through selection.

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Falcon 50mL Conical Centrifuge Tubes:Tubes:Centrifuge ...

Falcon 50mL Conical Centrifuge Tubes:Tubes:Centrifuge ...

Tested to withstand centrifugation of 16,000 RCF in a fully supported rotor with room temperature water. Corning™ Falcon 50mL Conical Centrifuge Tubes are ideal for cell centrifugation; pelleting; separation by density gradients.

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Centrifuge 5425/5425 R - Microcentrifuge - Eppendorf ...

Centrifuge 5425/5425 R - Microcentrifuge - Eppendorf ...

The Eppendorf Kit rotor F-45-18-11-Kit enables centrifugation of samples in 18 x spin columns and 1.5 or 2.0 mL tubes at a maximum speed of 18,565 x g. This unique rotor offers a solution for the most common problem associated with working with MiniPrep spin column kits, open tube lids shearing off during elution steps.

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RNA isolation using TRIzol reagent - Stanford University

RNA isolation using TRIzol reagent - Stanford University

Transfer the homogenate to 50 ml Oak Ridge Centrifuge tube and centrifuge at 12,000 g for 5-10 minutes at 4C. The resulting pellet contains extra-cellular membranes, polysaccharides and high molecular weight DNA while the supernatant contains RNA. 6. Remove the upper fat layer by using a Pasteur pipette hooked up to a vacuum flask.

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