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A protocol for recombinant protein quantification by ...

A protocol for recombinant protein quantification by ...

Apr 07, 2020 Using the information of protein load per band (Bio‐rad) for the bands of 100, 50, and 20 kDa, and the different loads in the SDS‐PAGE gel (10, 5, and 1 l), the amount of protein per band was positively correlated with the peak area using Microsoft Excel 2010 (Microsoft Company; p‐value .001, R 2 .98, Table 2). Ranging from 2.058 to ...

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Sex-specific chromatin remodelling safeguards ...

Sex-specific chromatin remodelling safeguards ...

Dec 08, 2021 Here, using an ultra-low-input native chromatin immunoprecipitation approach, we show that following DNA demethylation the gonadal primordial germ cells undergo remodelling of repressive histone ...

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PURIFICATION PROTOCOLS - wolfson.huji.ac.il

PURIFICATION PROTOCOLS - wolfson.huji.ac.il

Dec 14, 2017 GE-HEALTHCARE HiTrap Albumin IgG Depletion and Albumin IgG Depletion SpinTrap: prepacked columns for the depletion of albumin and IgG from human serum and plasma. For small-scale preparation of protein samples prior to downstream analyses such as 1-D or 2-D gel electrophoresis and mass spectrometry.

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Dexamethasone sensitizes to ferroptosis by glucocorticoid ...

Dexamethasone sensitizes to ferroptosis by glucocorticoid ...

Feb 02, 2022 Dexamethasone is widely used as an immunosuppressive therapy and recently as COVID-19 treatment. Here, we demonstrate that dexamethasone sensitizes to ferroptosis, a form of iron-catalyzed necrosis, previously suggested to contribute to diseases such as acute kidney injury, myocardial infarction, and stroke, all of which are triggered by glutathione (GSH) …

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Citrulline depletion by ASS1 is required for ...

Citrulline depletion by ASS1 is required for ...

Feb 03, 2022 Cells were lysed in a buffer containing 50 mM Tris-HCl at pH 7.4, 1 mM EDTA, 150 mM NaCl, 1% Triton X-100, proteinase inhibitors and phosphatase inhibitors, and incubated at 4C for 40-50 min, followed by centrifugation at 12000 rpm for 10 mins at 4C. 50 μL cell lysates was kept as input.

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A macrophage-hepatocyte glucocorticoid receptor axis ...

A macrophage-hepatocyte glucocorticoid receptor axis ...

Feb 03, 2022 Fasting metabolism and immunity are tightly linked; however, little is known about how immune cells engage in fasting homeostasis in healthy subjects. Using a cell-type-resolved genomics approach, Loft et al. demonstrate how glucocorticoid signaling in resident liver macrophages directly influences glucocorticoid signaling and ketogenesis in hepatocytes …

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Overview of blood components and their preparation - PMC

Overview of blood components and their preparation - PMC

INTRODUCTION. Blood is a lifesaving liquid organ. Whole blood is a mixture of cellular elements, colloids and crystalloids. As different blood components have different relative density, sediment rate and size they can be separated when centrifugal force is applied.[]In increasing order, the specific gravity of blood components is plasma, platelets, leucocytes (Buffy Coat [BC]) and …

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mTORC1 couples cyst(e)ine availability with GPX4 protein ...

mTORC1 couples cyst(e)ine availability with GPX4 protein ...

Mar 11, 2021 Introduction. Ferroptosis is an iron-dependent form of regulated cell death that is caused by excessive lipid peroxidation. It is morphologically, biochemically, and genetically distinct from other forms of regulated cell death 1, 2.Ferroptosis is inhibited by glutathione peroxidase 4 (GPX4), a glutathione peroxidase that utilizes reduced glutathione (GSH) as a …

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H3K9 dimethylation safeguards cancer cells against ...

H3K9 dimethylation safeguards cancer cells against ...

Mar 18, 2022 The sonication was performed using a Bioruptor with 30 cycles (15 s on/30 s off). The chromatin was quantified/qualified using Qubit (Life Technologies, Q32854) and the Agilent Bioanalyzer High-Sensitivity DNA kit (Agilent, 5067-4626), and 4% Drosophila spike-in was added to all samples. Immunoprecipitations were done with 7 μg of chromatin ...

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Supramolecular arrangement of protein in nanoparticle ...

Supramolecular arrangement of protein in nanoparticle ...

Nov 18, 2021 b, Biodistributions of variant crosslinked albumin NPs indicating uptake of albumin nanorods (n = 3 i.v.-LPS animals, n = 3 naive animals; red box, P 1 10 −10) and bovine albumin NPs (n = 3 ...

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KDM5B promotes immune evasion by recruiting SETDB1 to ...

KDM5B promotes immune evasion by recruiting SETDB1 to ...

Oct 20, 2021 Using an ICB-resistant YUMM1.7 murine melanoma model 18, we found that depletion of KDM5B not only significantly decreases the growth rate of YUMM1.7 tumours but also renders these tumours ...

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SLC25A39 is necessary for mitochondrial glutathione import ...

SLC25A39 is necessary for mitochondrial glutathione import ...

Oct 27, 2021 Target cells in 6-well tissue culture plates were infected in medium containing 8 g ml −1 of polybrene and a spin infection was performed by centrifugation at …

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Bradford Protein Assay - an overview ScienceDirect Topics

Bradford Protein Assay - an overview ScienceDirect Topics

The number of tPA molecules conjugated to the particle surface can be calculated using the Bradford protein assay (Bio-Rad Protein Assay Kit II, Bio-Rad, Hercules, CA). Initially, a standard curve is generated using bovine serum albumin (BSA). The BSA standard is dissolved in 20 ml of ultra pure water, resulting in a solution with 1.44 mg BSA/ml.

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