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Simple spectrophotometric assay for ... - BMC Biochemistry

Simple spectrophotometric assay for ... - BMC Biochemistry

Aug 03, 2018 The details of a precise, accurate, and sensitive spectrophotometric method for measuring catalase activity are presented here. The assay was established for biological samples and depends on the rapid formation of a stable and colored carbonato-cobaltate (III) complex. Samples exhibiting catalase activity are incubated with hydrogen peroxide solution for …

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Evaluation of Glucose Oxidase and Hexokinase Methods

Evaluation of Glucose Oxidase and Hexokinase Methods

Conclusion: ... for energy (1). Many analytical procedures are used to measure blood glucose concentration. In the past, analyses were often performed with relatively non specific methods that resulted in falsely . ... Plasma was got after centrifugation was used for analysis of glucose by glucose oxidase-peroxidase and hexokinase method.

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Pre-analytical Variables in Coagulation Testing Associated ...

Pre-analytical Variables in Coagulation Testing Associated ...

Feb 01, 2012 Pre-analytical Variables in Coagulation Testing Associated With Diagnostic Errors in Hemostasis ... Citrate anticoagulated plasma post single centrifugation ... and may also lead to a false conclusion of an elevated APTT. In extreme cases, microfiltration may even generate false (weak) positive LA findings. ...

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Pre-Analytical Requirements - PMC

Pre-Analytical Requirements - PMC

Introduction. A large proportion, possibly a majority, of errors in laboratory medicine occur in the pre-analytical phase of the testing process. 1 Hence in the assessment and evaluation of biochemical assays, pre-analytical factors need as thorough a consideration and investigation as the more traditional direct analytical factors. A key goal must be ‘correct first time, every time’.

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Spectrophotometry - an overview ScienceDirect Topics

Spectrophotometry - an overview ScienceDirect Topics

It is characterized by a wide analytical working range, and therefore sample dilution is avoided. Versatility, low cost, easy implementation, etc. are in general inherent to this technique. ... involving the factors of pH, centrifugation time, and buffer concentration. ... 5.5 Conclusion.

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Verification of quantitative analytical methods in medical ...

Verification of quantitative analytical methods in medical ...

Jun 05, 2021 Analytical specificity is referred to the measurement capability of an analytical method to exclude any interference in general, ... tube selection, and sample processing as centrifugation, transport and preservation should be assigned ... Conclusion. Several global workgroups exert much effort in establishing protocols and guidelines to rule ...

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Analytical Methods Used for the Detection and ...

Analytical Methods Used for the Detection and ...

Mar 21, 2022 Alone or coupled with other analytical techniques such as HPLC, GC, and TLC, it is used for the determination, qualitative or quantitative, of many organic substances like BZD [51, 69–71]. The variation of the potential imposed on the working electrode, as a function of time, can be linear continuous, differential with pulse, or in the form ...

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History of Cell Biology - Bitesize Bio

History of Cell Biology - Bitesize Bio

Nov 05, 2007 The cell theory, or cell doctrine, states that all organisms are composed of similar units of organization, called cells. The concept was formally articulated in 1839 by Schleiden Schwann and has remained as the foundation of modern biology.

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Qualitative and Quantitative Determination of ...

Qualitative and Quantitative Determination of ...

Oct 20, 2016 Analytical method is according to Ejikeme et al. . To each sample (0.30 g) weighed into a beaker was added 30 cm 3 of distilled water. After 24 hours of extraction, aqueous extract (10 cm 3) of each wood sample was boiled with 5 cm 3 of 1% aqueous hydrochloric acid. Deposit of red precipitate showed positive test. 2.4.3. Test for Saponin

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Qualitative and Quantitative Determination of ...

Qualitative and Quantitative Determination of ...

Oct 20, 2016 The phytochemical contents of some milled Nigerian softwood chips were carried out in a quest to evaluate their potentials as sources of alternative medicine as well as uses in other industrial applications. The qualitative and quantitative analysis were ascertained. Tannin was found in all the Nigerian softwoods examined with the highest quantities obtained ini …

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Solid-Phase Extraction - an overview ScienceDirect Topics

Solid-Phase Extraction - an overview ScienceDirect Topics

Rstem Keili, ... Chaudhery Mustansar Hussain, in Handbook of Nanomaterials in Analytical Chemistry, 2020. 5.3 Solid-phase extraction. SPE is an efficient tool for the sample preparation in chemistry. SPE was first applied in 1940s [48], and the rapid progresses in various SPE applications were initiated in 1970s.It is now successfully applied in different fields of science.

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