Aug 02, 2010 Centrifuge at 13,000 g for 30 minutes with the back of the tubes facing outwards for a consistent tube orientation in the rotor. Carefully aspirate and discard the supernatant. A pellet should be visible but can be very difficult to see at this stage. Work under a bright light source (e.g. desk lamp) to assist in visualizing the pellet.
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Get DetailsLet test tube sit 20 minutes to allow serum to separate (or centrifuge 3-5 minutes at 2000-3000-rpm). In the separated sample, serum will be on top. …
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Get DetailsPipet Filler, safety bulb 1 Sample cells ( For information about sample cells, adapters or light shields, refer to Instrument-specific information on page 1.) 2 Stoppers 2 ... Fill a centrifuge tube completely with sample and immediately cap the tube. 2. Put the tube in a centrifuge and run the centrifuge to separate the solids.
Get DetailsRelative density, or specific gravity, is the ratio of the density (mass of a unit volume) of a substance to the density of a given reference material. Specific gravity for liquids is nearly always measured with respect to water at its densest (at 4 C or 39.2 F); for gases, the reference is air at room temperature (20 C or 68 F). The term relative density is often preferred in ...
Get DetailsSep 16, 2014 We use a pipet bulb or a delivery tube to dispense the liquid. Never use your mouth to draw liquid into the pipet. 16. Glass Dropper Pipet A droper pipet is used to transfer a small volume of liquid, usually one drop at a time. On top of each pipet is a “rubber bulb” – never put your mouth on the dropper to provide suction 17.
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