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Extracting DNA Using Phenol-Chloroform - PacBio

Extracting DNA Using Phenol-Chloroform - PacBio

19. Spin for 20 minutes in a 4 C centrifuge at top speed. 20. Decant supernatant carefully without disturbing the pellet. 21. Wash by adding 300 L of 80% EtOH and vortex 3 times . 22. Spin for 15 minutes in a 4 C centrifuge at top speed. 23. Decant supernatant carefully without disturbing the pellet. 24.

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PowerSoil DNA Isolation Kit - Qiagen

PowerSoil DNA Isolation Kit - Qiagen

Alternatively, sterile DNA-Free PCR Grade Water may be used for elution from the silica Spin Filter membrane at this step (MO BIO Catalog# 17000-10). 21. Centrifuge at room temperature for 30 seconds at 10,000 x g. 22. Discard the Spin Filter . The DNA in the tube is now ready for any downstream application. No further steps are required.

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Quick Protocol for Extraction and Purification of Genomic DNA

Quick Protocol for Extraction and Purification of Genomic DNA

Apr 30, 2019 Centrifuge for 1 minute at maximum speed ( 12,000 x g) to elute the gDNA. Additional Resources you may find helpful: Monarch Genomic DNA Purification Kit Product Manual; Choosing Input Amounts for the Monarch Genomic DNA Purification Kit; Troubleshooting Guide for Genomic DNA Extraction Purification

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DNA Stable-Isotope Probing (DNA-SIP) - PMC

DNA Stable-Isotope Probing (DNA-SIP) - PMC

Aug 02, 2010 Using the DNA concentrations determined in step 2.3, calculate the required volume of extracted DNA that is required to provide 0.5 μg - 5 μg of DNA in the ultracentrifuge tubes. Combine extracted DNA (0.5 - 5 μg) with Gradient Buffer (see step 1.3) and 4.8 ml of 7.163 M CsCl to a total volume of ~6 ml in a sterile disposable 15-ml tube.

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BugBusterTM Protein Extraction Reagent

BugBusterTM Protein Extraction Reagent

BugBusterTM Protein Extraction Reagent United States Canada 800-207-0144 TB245 09/00 Novagen 3 Germany 0800 6931 000 United Kingdom 0800 622935 Or your local sales office 2. Resuspend the pellet from step 4 above in the same volume of BugBuster reagent that was

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Biotechnology 101 Protocol: DNA Extraction from Saliva ...

Biotechnology 101 Protocol: DNA Extraction from Saliva ...

By spinning the sample with centrifuge, we seperate the cell material from the DNA, which gives us a cleaner DNA sample. To spin the PCR tube with your sample (3) in the Bento Lab’s microcentrifuge, you will need to use the PCR tube adapter (1) that sits in a normal microcentrifuge tube (2) and converts it to fit a PCR tube.

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Centrifugation - Wikipedia

Centrifugation - Wikipedia

Centrifugation is a mechanical process which involves the use of the centrifugal force to separate particles from a solution according to their size, shape, density, medium viscosity and rotor speed. The denser components of the mixture migrate away from the axis of the centrifuge, while the less dense components of the mixture migrate towards the axis.

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Circulating tumor DNA - Wikipedia

Circulating tumor DNA - Wikipedia

Circulating tumor DNA (ctDNA) is tumor-derived fragmented DNA in the bloodstream that is not associated with cells. ctDNA should not be confused with cell-free DNA (cfDNA), a broader term which describes DNA that is freely circulating in the bloodstream, but is not necessarily of tumor origin. Because ctDNA may reflect the entire tumor genome, it has gained traction for its …

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CytoFLEX Research Flow Cytometry - Beckman

CytoFLEX Research Flow Cytometry - Beckman

CytoFLEX Flow Cytometer. The CytoFLEX Flow Cytometer, the first introduction to the CytoFLEX Platform, provides the performance you need in an easy to use system allowing you to focus on the science, not the instrumentation.Its superior sensitivity and resolution throughout all configurations give it the edge over other cytometry systems four times its size.

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DNeasy Plant Pro and Plant Kits - Qiagen

DNeasy Plant Pro and Plant Kits - Qiagen

For extraction of total cellular DNA from plant cells and tissues or fungi, or genomic DNA from plant cells, tissues and seeds ... making the DNA easier to redissolve. Centrifuge at 10,000–15,000 x g for 5–15 min at 4C. ... Recover the precipitated DNA by centrifugation at full speed in a microcentrifuge for 15–20 min;

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Ethanol precipitation of nucleic acids - OpenWetWare

Ethanol precipitation of nucleic acids - OpenWetWare

Jul 01, 2012 We tend to wash the DNA with 70% Ethanol after removing the first supernatant (the one containing sodium acetate and 100% Ethanol). Which means add 200-300l 70% Ethanol to the DNA-pellet. Then spin at full speed for 5mins @ 4C. Carefully remove supernatant. Proceed with drying. --Janosch; See also. Purification of DNA - overview of methods

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MG Scientific Distributor Laboratory Equipment Lab ...

MG Scientific Distributor Laboratory Equipment Lab ...

MG Scientific 8500 107th Street Pleasant Prairie WI 53158 800-343-8338

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Events - Beckman Coulter

Events - Beckman Coulter

not all products are available in all countries. product availability and regulatory status depends on country registration per applicable regulations

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Sorvall LYNX 4000 Superspeed Centrifuge

Sorvall LYNX 4000 Superspeed Centrifuge

Perform high-speed separations simply in shared lab settings with the Thermo Scientific Sorvall LYNX 4000 superspeed centrifuge with 4L capacity and performance up to 68,905 x g. Advanced rotor innovations including Thermo Scientific Auto-Lock rotor exchange, Thermo Scientific Auto-ID instant rotor

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Vi-CELL XR Cell Analyzer - Beckman Coulter

Vi-CELL XR Cell Analyzer - Beckman Coulter

Vi-CELL XR Cell Viability Analyzer. The Vi-CELL ™ XR Cell Viability Analyzer provides an automatic means to perform the Trypan Blue Dye Exclusion method, allowing users to load up to 9 samples at once for easy and automated cell analysis. Vi-CELL software offers pre-programmed and customizable analysis options for consistent and accurate analysis of simple cell systems …

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