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Extracting DNA Using Phenol-Chloroform - PacBio

Extracting DNA Using Phenol-Chloroform - PacBio

22. Spin for 15 minutes in a 4 C centrifuge at top speed. 23. Decant supernatant carefully without disturbing the pellet. 24. Repeat steps 21 – 23 for a seco nd 80% EtOH wash. 25. Quick spin on table top centrifuge to draw residual EtOH to the bottom. 26. Remove residual EtOH with a P20 pipette. Be careful not to disturb the pellet. 27.

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DNA - Isolation Protocols - The Jackson Laboratory

DNA - Isolation Protocols - The Jackson Laboratory

8. Spin in microfuge at top speed 5 minutes and transfer 0.5 ml of the upper phase to new microfuge tube. (Use P1000 for transfer, and draw the aqueous phase gently through tip several times after transfer if the DNA is still in large, gelatinous mass.) 9.

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PowerSoil DNA Isolation Kit - Qiagen

PowerSoil DNA Isolation Kit - Qiagen

Alternatively, sterile DNA-Free PCR Grade Water may be used for elution from the silica Spin Filter membrane at this step (MO BIO Catalog# 17000-10). 21. Centrifuge at room temperature for 30 seconds at 10,000 x g. 22. Discard the Spin Filter . The DNA in the tube is now ready for any downstream application. No further steps are required.

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Quick Protocol for Extraction and Purification of Genomic DNA

Quick Protocol for Extraction and Purification of Genomic DNA

Apr 30, 2019 Centrifuge for 1 minute at maximum speed ( 12,000 x g) to elute the gDNA. Additional Resources you may find helpful: Monarch Genomic DNA Purification Kit Product Manual; Choosing Input Amounts for the Monarch Genomic DNA Purification Kit; Troubleshooting Guide for Genomic DNA Extraction Purification

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DNA Stable-Isotope Probing (DNA-SIP) - PMC

DNA Stable-Isotope Probing (DNA-SIP) - PMC

Aug 02, 2010 Using the DNA concentrations determined in step 2.3, calculate the required volume of extracted DNA that is required to provide 0.5 μg - 5 μg of DNA in the ultracentrifuge tubes. Combine extracted DNA (0.5 - 5 μg) with Gradient Buffer (see step 1.3) and 4.8 ml of 7.163 M CsCl to a total volume of ~6 ml in a sterile disposable 15-ml tube.

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BugBusterTM Protein Extraction Reagent

BugBusterTM Protein Extraction Reagent

BugBusterTM Protein Extraction Reagent United States Canada 800-207-0144 TB245 09/00 Novagen 3 Germany 0800 6931 000 United Kingdom 0800 622935 Or your local sales office 2. Resuspend the pellet from step 4 above in the same volume of BugBuster reagent that was

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Biotechnology 101 Protocol: DNA Extraction from Saliva ...

Biotechnology 101 Protocol: DNA Extraction from Saliva ...

By spinning the sample with centrifuge, we seperate the cell material from the DNA, which gives us a cleaner DNA sample. To spin the PCR tube with your sample (3) in the Bento Lab’s microcentrifuge, you will need to use the PCR tube adapter (1) that sits in a normal microcentrifuge tube (2) and converts it to fit a PCR tube.

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Circulating tumor DNA - Wikipedia

Circulating tumor DNA - Wikipedia

Circulating tumor DNA (ctDNA) is tumor-derived fragmented DNA in the bloodstream that is not associated with cells. ctDNA should not be confused with cell-free DNA (cfDNA), a broader term which describes DNA that is freely circulating in the bloodstream, but is not necessarily of tumor origin. Because ctDNA may reflect the entire tumor genome, it has gained traction for its …

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CytoFLEX Research Flow Cytometry - Beckman

CytoFLEX Research Flow Cytometry - Beckman

CytoFLEX Flow Cytometer. The CytoFLEX Flow Cytometer, the first introduction to the CytoFLEX Platform, provides the performance you need in an easy to use system allowing you to focus on the science, not the instrumentation.Its superior sensitivity and resolution throughout all configurations give it the edge over other cytometry systems four times its size.

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Ethanol precipitation - Wikipedia

Ethanol precipitation - Wikipedia

During incubation DNA and some salts will precipitate from solution, in the next step this precipitate is collected by centrifugation in a microcentrifuge tube at high speeds (~12,000g). Time and speed of centrifugation has the biggest effect on DNA recovery rates. Again smaller fragments and higher dilutions require longer and faster ...

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DNeasy Plant Pro and Plant Kits - Qiagen

DNeasy Plant Pro and Plant Kits - Qiagen

For extraction of total cellular DNA from plant cells and tissues or fungi, or genomic DNA from plant cells, tissues and seeds ... making the DNA easier to redissolve. Centrifuge at 10,000–15,000 x g for 5–15 min at 4C. ... Recover the precipitated DNA by centrifugation at full speed in a microcentrifuge for 15–20 min;

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Ethanol precipitation of nucleic acids - OpenWetWare

Ethanol precipitation of nucleic acids - OpenWetWare

Jul 01, 2012 We tend to wash the DNA with 70% Ethanol after removing the first supernatant (the one containing sodium acetate and 100% Ethanol). Which means add 200-300l 70% Ethanol to the DNA-pellet. Then spin at full speed for 5mins @ 4C. Carefully remove supernatant. Proceed with drying. --Janosch; See also. Purification of DNA - overview of methods

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Current Nucleic Acid Extraction Methods and Their ...

Current Nucleic Acid Extraction Methods and Their ...

Jul 12, 2017 NAE methods encompass extraction of both DNA and RNA but can be more broadly characterized into chemically driven or solid-phase methods; both contain the four steps mentioned above [1, 4, 5]. In the next sections, we will review the working principle of and/or rationale for the main methods used nowadays in the biological and medical sciences.

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MG Scientific Distributor Laboratory Equipment Lab ...

MG Scientific Distributor Laboratory Equipment Lab ...

MG Scientific 8500 107th Street Pleasant Prairie WI 53158 800-343-8338

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Vi-CELL XR Cell Analyzer - Beckman Coulter

Vi-CELL XR Cell Analyzer - Beckman Coulter

Vi-CELL XR Cell Viability Analyzer. The Vi-CELL ™ XR Cell Viability Analyzer provides an automatic means to perform the Trypan Blue Dye Exclusion method, allowing users to load up to 9 samples at once for easy and automated cell analysis. Vi-CELL software offers pre-programmed and customizable analysis options for consistent and accurate analysis of simple cell systems …

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