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Reassessment of Exosome Composition - PMC

Reassessment of Exosome Composition - PMC

Apr 04, 2019 The principal fractions containing the CD63/CD81/CD9 proteins after high-resolution density gradient centrifugation indicates that the majority of classical exosomes have buoyant densities in iodixanol-PBS from 1.08–1.13 g/ml, …

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Cryo-ET of Env on intact HIV virions reveals structural ...

Cryo-ET of Env on intact HIV virions reveals structural ...

Feb 04, 2022 However, in our structure, only partial density for the HR1-C helix can be observed corresponding to gp41 residues 570–577 and 582–595 (Figure 5A). Classification of Env sub-volumes with a tight cylindrical mask for the gp41 region did not yield any subset with higher density for HR1-C helices in our data.

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ICAM-1-mediated adhesion is a prerequisite for exosome ...

ICAM-1-mediated adhesion is a prerequisite for exosome ...

Feb 07, 2022 For iodixanol density gradient centrifugation, a discontinuous iodixanol gradient (5%, 10%, 20% and 40%) was made by diluting 60% OptiPrep aqueous iodixanol with 0.25 M sucrose in 10 mM Tris. Exosomes purified by differential centrifugation were further loaded on the top of the discontinuous iodixanol gradient and then centrifuged at 100,000 g ...

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Rapid and simplified purification of recombinant adeno ...

Rapid and simplified purification of recombinant adeno ...

Iodixanol (OptiPrep) ultrahigh speed density gradient centrifugation is a similar method developed for AAV purification, with a higher viral recovery compared to the CsCl method, but the Iodixanol reagent is expensive and the method still remains relatively time-consuming; the latter drawback prevents its use for assessing quickly gene ...

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Iodixanol - Wikipedia

Iodixanol - Wikipedia

It is sold under the trade name Visipaque; it is also sold as a density gradient medium under the name OptiPrep. Visipaque is commonly used as a contrast agent during coronary angiography. It is the only iso-osmolar contrast agent, with an osmolality of 290 mOsm/kg H 2 O, the same as blood. It is sold in 2 main concentrations 270 mgI/ml and 320 ...

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Methods of isolation and purification of outer membrane ...

Methods of isolation and purification of outer membrane ...

Jan 01, 2015 Density gradient centrifugation. Due to the lipid content the density of OMV is lower than that of soluble secreted proteins, flagella, pili etc. and thus during centrifugation in density gradient OMV migrate to lighter fractions (Horstman and …

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AAV Purification by Iodixanol Gradient ... - Addgene

AAV Purification by Iodixanol Gradient ... - Addgene

Jan 17, 2018 In medicine, iodixanol can be used as an intravenous isomolar contrast agent. In research, iodixanol is used as an isomolar density gradient medium suitable for virus purification and isolation of cells, organelles, lipoproteins, and macromolecules. Importantly, iodixanol is inert and non-toxic to mammalian cells.

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A comparison of methods for the ... - Scientific Reports

A comparison of methods for the ... - Scientific Reports

Jan 23, 2020 Iodixanol density gradient centrifugation Flow cytometry analysis The flow cytometer was equipped with 375 nm, 405 nm, 488 nm, 561 nm, 638 nm, and 808 nm lasers to detect up to 21 fluorescence ...

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Subcellular Fractionation -

Subcellular Fractionation -

Nov 14, 2021 Sequential centrifugation can be used to prepared endosomes. For example, neuronal endosomes were obtained through first lyzing neurons with 20 times of syringe aspiration in lysis buffer (250 mM sucrose, 50 mM Tris-HCl, pH 7.4, 5 mM MgCl2, 1mM EDTA, 1mM EGTA) along with protease inhibitor mixture and then going through sequential …

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Product Types - Products - Stemcell Technologies

Product Types - Products - Stemcell Technologies

OptiPrep 1 item ... Centrifugation Tubes 3 items ... Density Gradient Media . ELISAs . Genome Editing and Molecular Tools ...

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