A three-phase liquid-liquid-solid centrifuge uses extremely high g-force (over 7,000 Gs) to separate the entrapped water and fine particles down to a 1-micron level in a single pass. Industrial Wash-Water Centrifuge. Alfa Laval Wash Water Centrifuge.
Get DetailsAug 28, 2018 8.8. Centrifuge the samples at 12,000 x g for 15 min at 4C. 8.9. Transfer the upper aqueous phase carefully without disturbing the inter-phase into a new eppendorf. 8.10. Add 0.5 mL of isopropanol alcohol to the aqueous phase and …
Get DetailsDec 13, 2007 This protocol for solid-phase peptide synthesis (SPPS) is based on the widely used Fmoc/tBu strategy, activation of the carboxyl groups by aminium-derived coupling reagents and use of PEG-modified ...
Get DetailsDemand more flexibility. Eaton's PowerXL DG1 general purpose drives are engineered for today’s demanding commercial and industrial applications. With an industry leading energy efficiency algorithm, high short-circuit current rating and robust design, the DG1 offers customers increased efficiency, safety and reliability.
Get DetailsDesigned for Phase Converters. American Rotary's custom designed generator uses only 1/3 of the starting current to get the generator started and unlike traditional off the shelf three phase motors they are capable of running 24/7 with no duty cycle.
Get DetailsIn a two-phase decanter, the liquid level is regulated by dam plates. When operating in a three-phase mode, each phase discharges over a set of dam plates into separate baffled compartments in the casing. In certain applications, a centripetal pump discharge that utilizes the pressure head developed by the rotating liquid phase is used to pump
Get DetailsIncreasing the pressure drives the water into the higher density phase, which causes melting. Another interesting though not unusual feature of the phase diagram is the point where the solid–liquid phase line meets the liquid–gas phase line. The intersection is referred to as the triple point. At the triple point, all three phases can coexist.
Get DetailsJan 04, 2021 How does a laboratory centrifuge work? Artwork: Inside a typical high-speed laboratory centrifuge. From US Patent 2,699,289: High-speed centrifuge by Robert R. Allen, Custom Scientific Instruments Inc., patented January 11, 1955, courtesy of US Patent and Trademark Office. If you've studied biology or chemistry in school, you've probably used a …
Get DetailsJan 15, 2021 A centrifuge such as this can spin at up to 15,000 rpm to facilitate separation of the different phases of the extraction. It is also used to precipitate the DNA after the salts are washed away with ethanol and or isopropanol.
Get DetailsOct 02, 2010 Add 70% lead acetate to the filtrate and centrifuge at 5000rpm/10 min. Further centrifuge the supernatant by adding 6.3% Na 2 CO 3 at 10000 rpm/10min. Dry the retained supernatant and redissolved in chloroform and use for chromatography.
Get DetailsThe disc stack centrifuge is a versatile device, which may be used for separating solid/liquid mixtures in continuous, semi-continuous and batch configurations (see Figures 1.12 and 1.13).All except some batch-operated machines are able to handle toxic, flammable and volatile feeds at throughputs up to 200 m 3 h −1. Liquid-liquid mixtures can be separated and with more …
Get DetailsThe homogenized tissue suspension is centrifuge under 4℃ with 5000 rpm for 5 min to get the upper clear solution. Take 100 μL upper solution into a centrifugal tube and then add 300 μL acetonitrile to precipitate protein, and then centrifuge under 4℃ with 5000 rpm for 5 min to get upper clear solution.
Get DetailsThe three enrichment processes described below have different characteristics. Diffusion is flexible in response to demand variations and power costs but is very energy-intensive. With centrifuge technology it is easy to add capacity with modular expansion, but it is inflexible and best run at full capacity with low operating cost.
Get DetailsThe Zippe-type centrifuge is a gas centrifuge designed to enrich the rare fissile isotope uranium-235 (235 U) from the mixture of isotopes found in naturally occurring uranium compounds. The isotopic separation is based on the slight difference in mass of the isotopes. The Zippe design was originally developed in the Soviet Union by a team led by 60 Austrian and …
Get DetailsThree types of processes can be utilized for liquefaction. Figure 7.13 shows the three options. Process 1 is where the α-amylase is added and the material is incubated at 85-95C. Process 2 has the mash in the jet cooker at 105-120C for 2-7 minutes, then flows to a flash tank at 90C. α-Amylase is added three hours later.
Get DetailsUsing a purifier the light liquid phase is typically the large fraction which is meant to cleaned. Concentrator The Alfa Laval concentrator is a centrifugal separator designed to separate three different phases, one solid phase and two liquid phases of different densities, and clean the densest/heaviest liquid phase.
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