Apr 26, 2021 Cells were cultured in an incubator (Thermo Fisher Scientific) ... IL-15-NP solution was applied to the rotary evaporator for dichloromethane evaporation. Next, the emulsion was centrifuged to ...
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Get DetailsFeb 09, 2022 Introduction. Diabetes mellitus (DM) is a chronic metabolic disease attributed to hyperglycemia, hyperlipidemia, and hyperaminoacidemia, along with insulin insufficiency, insulin-receptor resistance, or both. 1 It affects nearly 10% of the global population. Despite the introduction of modern hypoglycemic agents, secondary complications remain a major medical …
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Get DetailsJan 06, 2022 The 2A-tagged proteins were separated on Novex 8–16% Tris-Glycine Gels (Thermo Fisher Scientific) and blotted onto a PVDF membrane. For detection, an anti-2A antibody (1:5,000, ABS31, Merck) was ...
Get DetailsMar 21, 2022 A rotary evaporator at 300 mbar and 35 C with 135 rpm was used to evaporate the residual solvent. After the evaporation, a wax-like residue was obtained. ... from blood samples, ≥200 μl of blood was collected in heparinized tubes (Fisher Scientific) and immediately diluted with 3 ml of PBS. 3 ml of Histopaque 1083 (Sigma-Aldrich) was then ...
Get DetailsMolecular gastronomy is the scientific approach of nutrition from primarily the perspective of chemistry.The composition (molecular structure), properties (mass, viscosity, etc) and transformations (chemical reactions, reactant products) of an ingredient are addressed and utilized in the preparation and appreciation of the ingested products.It is a branch of food …
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Get DetailsThen the solvent is removed by a rotary evaporator and is further precipitated in diethyl ether. Yield: 0.28 g (58%). Synthesis of the PEG-b-IR. Triethylamine (200 μL) was added to a solution of PEG-b-PNH 2 (0.20 g) in DMF and the mixture was stirred for 30 min. The IR 780 (0.03 g, 45 μmol) was added to the mixture and the solution was ...
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