Another new sonicator available from BioSpec Products is the cordless handheld Sonozap Ultrasonic Homogenizer. The 1/8 in. diameter auto-tuned probe is ideal for small samples of 0.3–5.0 ml. Pressure Biosciences, Inc. ( www.pressurebiosciences.com ) has developed the Barocycler™, bench top, and PCT Shredder, hand held, sample preparation ...
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Get DetailsReduce the viscosity of the suspension by sonicating 5 min at full power with 50% duty cycle (on for 5 sec, off for 5 sec) using an ultrasonic homogenizer, as described in UNIT 6.2, step 5. 5 Unbroken cells, large cellular debris, and the inclusion body protein will be pelleted.
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